Actividad gastroprotectora de la fracción butanólica obtenida de hojas de Capraria biflora L.
Fecha
2017
Autores
Mastrapa Rodriguez, Tahimy
Título de la revista
ISSN de la revista
Título del volumen
Editor
Universidad Central “Marta Abreu” de Las Villas. Facultad de Química Farmacia. Departamento de Farmacia
Resumen
Se determinó el efecto gastroprotector de una fracción butanólica a partir del extracto acuoso de Capraria biflora L., especie comúnmente empleada en patologías crónicas asociadas a procesos inflamatorios. Dicha fracción es rica en compuestos fenólicos y le ha sido estudiada su actividad antinflamatoria por lo que pudiera comportarse como protectora de la mucosa gástrica. Para el estudio de la actividad gastroprotectora se evaluaron dos técnicas de producción de úlceras agudas utilizando como agentes ulcerogénicos al etanol absoluto (1 mL/250g) y la indometacina (50 mg/Kg) y una técnica de úlcera subaguda por indometacina en la cual se determinó la actividad de la enzima superóxido dismutasa. En el diseño experimental se utilizaron dosis de 50, 100 y 200 mg/Kg de peso y fueron empleados como controles positivos el omeprazol 20 mg/Kg (modelo de úlcera por etanol absoluto) y la ranitidina 100 mg/Kg (modelo de úlcera aguda y subaguda por indometacina). La fracción butanólica mostró efecto gastroprotector en los modelos agudos estudiados con grado de ulceración y porcientos de inhibición en la dosis de 200 mg/Kg similares a los controles positivos empleados para un p≤0,05. Similares resultados fueron obtenidos en el modelo de úlcera subaguda por indometacina, donde nuevamente la dosis de 200 mg/Kg mostró resultados comparables a la ranitidina. La administración de la fracción butanólica aumentó los valores de la enzima superóxido dismutasa con respecto al control negativo para un p≤0,05. La fracción butanólica a partir del extracto acuoso de Capraria biflora L. presenta actividad gastroprotectora en los modelos seleccionados.
Presently study is proven the gastroprotective effect of a butanol fraction from the aqueous extract of Capraria biflora L. This species it commonly used in chronic pathologies associated to inflammatory processes. The butanol fraction is rich in phenolic compound and its antiinflammatory activity has been studied. This can suggested that it could behave as gastric protector of the mucous. Were evaluated, for the study of gastroprotective activity, two techniques of production of acute ulcers using indomethacin (50mg/Kg) and absolute ethanol (1ml/250g) as ulcerogenic agents. Also a technique of subacute ulcers for indomethacin was developed and it was determined of the enzyme superoxide dismutase activity. In the experimental design dose of 50, 100 and 200 mg/Kg of weight were used and they were employees as positive controls the omeprazole 20 mg/Kg (of absolute ethanol model) and the ranitidine 100 mg/Kg (acute and subacute indomethacin model). The butanol fraction showed gastroprotective effect in the models studied with similar ulceration grade and inhibition percents in the dose of 200 mg/Kg, to the controls positive employees for a p≤0,05. Similar results were obtained in the subacute indomethacin model, where the dose of 200 mg/Kg showed results comparable to the ranitidine again. The administration of the butanol fraction increased the values of the enzyme superoxide dismutase with regard to the negative control for a p ≤0,05. The butanol fraction from the aqueous extract of Capraria biflora L. presents gastroprotective activity in the selected models.
Presently study is proven the gastroprotective effect of a butanol fraction from the aqueous extract of Capraria biflora L. This species it commonly used in chronic pathologies associated to inflammatory processes. The butanol fraction is rich in phenolic compound and its antiinflammatory activity has been studied. This can suggested that it could behave as gastric protector of the mucous. Were evaluated, for the study of gastroprotective activity, two techniques of production of acute ulcers using indomethacin (50mg/Kg) and absolute ethanol (1ml/250g) as ulcerogenic agents. Also a technique of subacute ulcers for indomethacin was developed and it was determined of the enzyme superoxide dismutase activity. In the experimental design dose of 50, 100 and 200 mg/Kg of weight were used and they were employees as positive controls the omeprazole 20 mg/Kg (of absolute ethanol model) and the ranitidine 100 mg/Kg (acute and subacute indomethacin model). The butanol fraction showed gastroprotective effect in the models studied with similar ulceration grade and inhibition percents in the dose of 200 mg/Kg, to the controls positive employees for a p≤0,05. Similar results were obtained in the subacute indomethacin model, where the dose of 200 mg/Kg showed results comparable to the ranitidine again. The administration of the butanol fraction increased the values of the enzyme superoxide dismutase with regard to the negative control for a p ≤0,05. The butanol fraction from the aqueous extract of Capraria biflora L. presents gastroprotective activity in the selected models.
Descripción
Palabras clave
Capraria biflora L., Plantas Medicinales, Estómago - Fisiopatología, Úlcera Péptica