Actividad antiinflamatoria in silico e in vivo de yucagenina presente en Agave brittoniana subps. brachypus (Trel.) A. Álvarez
Fecha
2018-05
Autores
Resino Ruiz, Dayana
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Editor
Universidad Central "Marta Abreu" de Las Villas.Facultad de Ciencias Agropecuarias.Departamento de Biología
Resumen
La especie Agave brittoniana subsp. brachypus (Trel.) A. Álvarez presenta actividad
antiinflamatoria en biomodelos experimentales, además se ha comprobado la
presencia de yucagenina como sapogenina mayoritaria. Sin embargo no existen
estudios que comprueben si este metabolito es el principal responsable del efecto
antiinflamatotio observado en la especie. Se realizaron estudios experimentales in
silico e in vivo para comprobar el efecto antiinflamatorio de la yucagenina presente
en esta especie. La predicción de las propiedades antiinflamatorias fue realizada
utilizando los programas TOMOCOMD, STATISTICA y WEKA, para obtener un
modelo computacional que permitió identificar los compuestos con potencial acción
antiinflamatoria. La actividad antiinflamatoria in vivo fue evaluada empleando modelo
agudo de inducción de edema por carragenina. Se formaron cinco grupos
experimentales: fracción rica en yucagenina (20mg/kg), fracción de sapogeninas
(50mg/kg), diosgenina patrón (20mg/kg) indometacina y control negativo. Los
resultados del estudio in silico demostraron la obtención de un modelo eficaz para la
detección de moléculas con potencial acción antiinflamatoria. Entre las sapogeninas
identificadas por el modelo como potenciales antiinflamatorias se encontró la
yucagenina. El modelo agudo in vivo corroboró que todas las fracciones de
sapogeninas evaluadas mostraron propiedades antiinflamatorias elevadas, incluso
mayores que la indometacina. La fracción rica en yucagenina desde la primera hora
inhibió la formación del edema. Concluimos que las sapogeninas presentes en Agave
brittoniana Trel, especialmente la yucagenina como sapogenina mayoritaria,
presenta potente actividad antiinflamatoria.
Agave brittoniana subsp. brachypus (Trel.) A. Álvarez presents anti-inflammatory activity in experimental biomodels. It has also been proven the presence of yucagenin as majority sapogenin. However, studies don't exist that check if this metabolite is the main responsible for the anti-inflammatory effect observed in this specie. Two experimental studies, in silico and in vivo were carried out to evaluate the antiinflammatory effect of yucagenin, present in the Agave brittoniana subsp. brachypus. The prediction of anti-inflammatory properties was performed using the programs TOMOCOMD, STATISTICA and WEKA, to develop a computational model useful to identify compounds with potential anti-inflammatory action. The anti-inflammatory activity in vivo was evaluated using an acute model the carrageenan-induced rat paw edema test. Thirty animals were used divided into five experimental groups: fraction rich in yucagenin (20mg / kg), fraction of sapogenins (50mg / kg), diosgenin standard (20mg / kg) indomethacin and negative control. From the in silico study we obtaining a good model to predict of anti-inflammatory activity of molecules. Among the sapogeninas identified with this model, yucagenin was found as potential antiinflammatory. The acute in vivo model showed that all the sapogenin fractions evaluated exhibited high anti-inflammatory properties, even higher than indomethacin. The fraction rich in yucagenin inhibited the formation of edema from the first hour. We conclude that the sapogenins present in Agave brittoniana Trel, especially the yucagenin as a major sapogenin, have potent anti-inflammatory activity.
Agave brittoniana subsp. brachypus (Trel.) A. Álvarez presents anti-inflammatory activity in experimental biomodels. It has also been proven the presence of yucagenin as majority sapogenin. However, studies don't exist that check if this metabolite is the main responsible for the anti-inflammatory effect observed in this specie. Two experimental studies, in silico and in vivo were carried out to evaluate the antiinflammatory effect of yucagenin, present in the Agave brittoniana subsp. brachypus. The prediction of anti-inflammatory properties was performed using the programs TOMOCOMD, STATISTICA and WEKA, to develop a computational model useful to identify compounds with potential anti-inflammatory action. The anti-inflammatory activity in vivo was evaluated using an acute model the carrageenan-induced rat paw edema test. Thirty animals were used divided into five experimental groups: fraction rich in yucagenin (20mg / kg), fraction of sapogenins (50mg / kg), diosgenin standard (20mg / kg) indomethacin and negative control. From the in silico study we obtaining a good model to predict of anti-inflammatory activity of molecules. Among the sapogeninas identified with this model, yucagenin was found as potential antiinflammatory. The acute in vivo model showed that all the sapogenin fractions evaluated exhibited high anti-inflammatory properties, even higher than indomethacin. The fraction rich in yucagenin inhibited the formation of edema from the first hour. We conclude that the sapogenins present in Agave brittoniana Trel, especially the yucagenin as a major sapogenin, have potent anti-inflammatory activity.
Descripción
Palabras clave
Agave Brittoniana, Carragenina, In Silico, Yucagenina