Evaluación del efecto hipolipemiante in vivo de saponinas y sapogeninas extraídas de la especie Agave brittoniana subps brachypus
Fecha
2018
Autores
López González, Beatríz
Título de la revista
ISSN de la revista
Título del volumen
Editor
Universidad Central “Marta Abreu” de Las Villas. Facultad de Química Farmacia. Departamento de Farmacia
Resumen
Se realizó un estudio experimental fitoquímico de las fracción n-butanólica del
extracto hidroalcohólico de A. brittoniana corroborando la presencia de saponinas y
azúcares reductores, metabolitos potencialmente activos desde el punto de vista
farmacológico. Al polvo seco, proveniente del material vegetal, y al extracto
hidroalcohólico se le realizó la comprobación de las características organolépticas
y físico-químicas, evidenciando que existen semejanzas en estudios previos. Se
evaluó la actividad antilipidémica por modelo in vivo de hiperlipidemia inducida por
P338 de una mezcla de sapogeninas provenientes de la planta, su fracción rica en
yucagenina (FRY) y de diosgenina patrón, a dosis de 50, 20 y 20 mg/kg de peso,
respectivamente. Se encontró la mezcla de sapogeninas manifestó actividad
hipocolesterolémica superior a la atorvastatina. Ninguna de las sustancias
estudiadas mostró actividad hipotrigliceridémica en este modelo. Se evaluó la
actividad antilipidémica por modelo in vivo de hiperlipidemia con el modelo de
ratones apoE-/- de la fracción n-butanólica y la mezcla de sus sapogeninas, a dosis
de 50 mg/kg. Las saponinas provenientes de la fracción n-butanólica del extracto
hidroalcohólico de hojas de A. brittoniana mostraron actividad hipotrigliceridémica,
en relación con la atorvastatina y provocaron pérdida de peso en el grupo de
animales tratados. Ninguna de las sustancias evaluadas por el modelo de ratones
apoE-/- mostró actividad hipocolesterolémica.
An experimental phytochemical study of the n-butanolic fraction of the hydroalcoholic extract of A. brittoniana was carried out, corroborating the presence of saponins and sugars reducing, potentially active metabolites from the pharmacological point of view. The dry powder, from the vegetable material, and the hydroalcoholic extract was checked for organoleptic and physico-chemical characteristics, evidencing that similarities exist in previous studies. The antilipidemic activity was evaluated by in vivo model of hyperlipidemia induced by P338 of a mixture of sapogenins from the plant, its fraction rich in yucagenin (FRY) and diosgenin standard, at doses of 50, 20 and 20 mg/kg of weight, respectively. The mixture of sapogenins was found to have hypocholesterolemic activity superior to atorvastatin. None of the substances studied showed hypotriglyceridemic activity in this model. Antilipidemic activity was evaluated by in vivo model of hyperlipidemia with the model of apoE- mice of the n-butanolic fraction and the mixture of its sapogenins, at doses of 50 mg/kg of weight. The saponins from the n-butanolic fraction of the hydroalcoholic extract of A brittoniana leaves showed hypotriglyceridemic activity, in relation to atorvastatin and caused weight loss in the group of treated animals. None of the substances evaluated by the apoE- mouse model.
An experimental phytochemical study of the n-butanolic fraction of the hydroalcoholic extract of A. brittoniana was carried out, corroborating the presence of saponins and sugars reducing, potentially active metabolites from the pharmacological point of view. The dry powder, from the vegetable material, and the hydroalcoholic extract was checked for organoleptic and physico-chemical characteristics, evidencing that similarities exist in previous studies. The antilipidemic activity was evaluated by in vivo model of hyperlipidemia induced by P338 of a mixture of sapogenins from the plant, its fraction rich in yucagenin (FRY) and diosgenin standard, at doses of 50, 20 and 20 mg/kg of weight, respectively. The mixture of sapogenins was found to have hypocholesterolemic activity superior to atorvastatin. None of the substances studied showed hypotriglyceridemic activity in this model. Antilipidemic activity was evaluated by in vivo model of hyperlipidemia with the model of apoE- mice of the n-butanolic fraction and the mixture of its sapogenins, at doses of 50 mg/kg of weight. The saponins from the n-butanolic fraction of the hydroalcoholic extract of A brittoniana leaves showed hypotriglyceridemic activity, in relation to atorvastatin and caused weight loss in the group of treated animals. None of the substances evaluated by the apoE- mouse model.
Descripción
Palabras clave
Plantas Medicinales, Extracto Hidroalcohólico, Agave Brittoniana Subps Brachypus, Hiperlipidemia, Epidemiología, Diagnóstico