Evaluación en modelos experimentales del efecto antiinflamatorio de extractos de Agave brittoniana Trel subespecie Brachypus
Fecha
2016-07
Autores
Martín Monteagudo, Dalys
Título de la revista
ISSN de la revista
Título del volumen
Editor
Universidad Central “Marta Abreu” de Las Villas. Facultad de Química Farmacia. Departamento de Farmacia
Resumen
Se realizó un estudio experimental fitoquímico del extracto hidroalcohólico de Agave brittoniana Trel subespecie brachypus (A. brittoniana) y farmacológico de los extractos hidroalcohólico y butanólico de Agave brittoniana con el objetivo de comprobar el efecto antiinflamatorio de los mismos en modelos experimentales de inflamación. El material vegetal recolectado se sometió a un estudio de secado corroborando que el método que emplea calor artificial en la estufa es el adecuado para procesar esta planta. Se realizó la caracterización físico-química de la droga seca y del extracto hidroalcohólico y se comprobó la presencia de saponinas y azúcares reductores como metabolitos mayoritarios. Se estudió la actividad antiinflamatoria de los extractos butanólico (crudo de saponinas) a 25, 50 y 100 mg/kg e hidroalcohólico a 100, 300 y 600 mg/kg. El modelo agudo de inducción de edema plantar por -carragenina, mostró que los grupos correspondientes a las dosis de 50 y 100 mg/kg del crudo de saponinas y a los 100 mg/kg del extracto hidroalcohólico mostraron los menores valores de inflamación y mayores porcentajes de inhibición, a las 3 y 4 horas de tratamiento. Según modelo crónico de granuloma inducido por discos de algodón, los extractos obtenidos a partir del A. brittoniana, exceptuando 600 mg/kg del extracto hidroalcohólico, presentan actividad antiinflamatoria en procesos crónicos, al reducir el peso seco del granuloma y por tanto reducir la formación de tejido granulomatoso
A phytochemical study and pharmacological experimental hydroalcoholic extract of Agave brittoniana Trel subspecies brachypus (A. brittoniana) hydroalcoholic and butanol extracts of Agave brittoniana in order to check the anti-inflammatory effect in experimental models of inflammation was performed. The plant material collected was subjected to a drying study confirming that the method using artificial heat on the stove is the right to process this plant. physico-chemical dry hydroalcoholic extract drug and characterization was performed and the presence of saponins and reducing sugars as major metabolites were found. antiinflammatory activity of butanol extracts (crude saponin) at 25, 50 and 100 mg / kg hydroalcoholic 100, 300 and 600 mg / kg was studied. Acute induction model plantar edema by -carrageenan showed that the groups corresponding to doses of 50 and 100 mg / kg crude saponins and 100 mg / kg of hydroalcoholic extract showed the lowest values of inflammation and higher percentages inhibition at 3 and 4 hours of treatment. According chronic model of granuloma induced by cotton pads, the extracts obtained from A. brittoniana except 600 mg / kg of hydroalcoholic extract, exhibit antiinflammatory activity in chronic processes, by reducing the dry weight of the granuloma and thus reduce the formation granulomatous tissue
A phytochemical study and pharmacological experimental hydroalcoholic extract of Agave brittoniana Trel subspecies brachypus (A. brittoniana) hydroalcoholic and butanol extracts of Agave brittoniana in order to check the anti-inflammatory effect in experimental models of inflammation was performed. The plant material collected was subjected to a drying study confirming that the method using artificial heat on the stove is the right to process this plant. physico-chemical dry hydroalcoholic extract drug and characterization was performed and the presence of saponins and reducing sugars as major metabolites were found. antiinflammatory activity of butanol extracts (crude saponin) at 25, 50 and 100 mg / kg hydroalcoholic 100, 300 and 600 mg / kg was studied. Acute induction model plantar edema by -carrageenan showed that the groups corresponding to doses of 50 and 100 mg / kg crude saponins and 100 mg / kg of hydroalcoholic extract showed the lowest values of inflammation and higher percentages inhibition at 3 and 4 hours of treatment. According chronic model of granuloma induced by cotton pads, the extracts obtained from A. brittoniana except 600 mg / kg of hydroalcoholic extract, exhibit antiinflammatory activity in chronic processes, by reducing the dry weight of the granuloma and thus reduce the formation granulomatous tissue
Descripción
Palabras clave
Agave brittoniana Trel, Estudios Fitoquímicos, Antiinflamatorio, Extracto Hidroalcohólico, Plantas Medicinales