Efficient direct shoot organogenesis, genetic stability and secondary metabolite production of micropropagated Digitalis purpurea L.

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dc.contributor.authorPérez Alonso, Naivy Lisbet
dc.contributor.authorMartín, Randel
dc.contributor.authorCapote, Alina
dc.contributor.authorPérez, Anabel
dc.contributor.authorKairuz Hernández Díaz, Elizabeth
dc.contributor.authorRojas, Luis
dc.contributor.authorJiménez, Elio
dc.contributor.authorQuiala, Elisa
dc.contributor.authorAngenon, Geert
dc.contributor.authorGarcia-Gonzales, Rolando
dc.contributor.authorChong Pérez, Borys
dc.contributor.departmentUniversidad Central “Marta Abreu” de Las Villas, Facultad de Ciencias Agropecuarias, Carretera a Camajuaní km 5,5, Santa Clara, Villa Clara, CP 54830, Cubaen_US
dc.contributor.departmentInstituto de Biotecnología de las Plantas, Universidad Central “Marta Abreu” de Las Villas, Carretera a Camajuaní km 5,5, Santa Clara, Villa Clara, CP 54830, Cubaen_US
dc.contributor.departmentFlorida Crystals Corp, 25550 State Road 880 Atlantic Sugar Mill Rd, Belle Glade, FL 33430, USAen_US
dc.contributor.departmentInstituto Nacional de Investigaciones Agropecuarias, INIAP Av. Eloy Alfaro N30-350 y Amazonas, Quito, Ecuadoren_US
dc.contributor.departmentLaboratory of Plant Genetics, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgiumen_US
dc.contributor.departmentSociedad de Investigación y Servicios BioTECNOS Ltda, Camino a Pangal km 2,5, San Javier, Chileen_US
dc.contributor.departmentBotanical Solutions SpA, Ave. Quilin 3550, Santiago de Chile, Chileen_US
dc.date.accessioned2018-11-01T17:57:44Z
dc.date.available2018-11-01T17:57:44Z
dc.date.issued2018-03-06
dc.description.abstractCardiovascular and cancer diseases are the first causes of death in the world. Digitalis purpurea L. is a medicinal plant that produces secondary metabolites, like digoxin and digitoxin, which are employed in therapies against heart failure. Moreover, anticancer and antiviral properties of these metabolites have recently been described. The present work details a method to obtain in vitro plants of D. purpurea from leaf segments through direct organogenesis. A reliable and efficient plant induction system was established by optimizing the concentration of naphthaleneacetic acid (NAA) and 6-benzylaminopurine (6-BAP). The highest frequency of shoot regeneration (98.5%) with an average number of shoots per leaf segment of 18.9 was achieved via direct organogenesis from leaf segment on MS medium containing 0.54 μM NAA + 13.2 μM 6-BAP. Additionally, Random Amplified Polymorphic DNA (RAPD) analysis showed 100% monomorphic bands, which indicated genetic stability of the obtained plants. Moreover, leaf powder of de novo regenerated plants fulfills the quality specifications of the British Pharmacopoeia and HPLC analysis revealed the presence of digoxin (22.6 μg gDW−1) and digitoxin (220.7 μg gDW−1) without significant differences in contents between de novo regenerated and mother plants. An efficient in vitro propagation protocol via direct organogenesis and genetic stability assessment of D. purpurea for obtaining leaf powder with quality for the use as raw material have thus been described. The protocol also provides an effective means for several approaches in biotechnology and breeding programs, in order to produce pharmaceutically interesting cardenolides.en_US
dc.identifier.doi10.1016/j.indcrop.2018.02.067en_US
dc.identifier.urihttps://dspace.uclv.edu.cu/handle/123456789/10155
dc.language.isoen_USen_US
dc.relation.journalIndustrial Crops and Productsen_US
dc.rightsEste documento es Propiedad Patrimonial de Elsevier y se deposita en este Repositorio solo con fines académicos y exclusivamente para usuarios de la UCLV hasta tanto sea liberado por la revista: Industrial Crops and Products respetando la legislación vigente en Cuba sobre derecho de Autor y la política de acceso de la mencionada publicación periódica.en_US
dc.rights.holderElsevieren_US
dc.source.endpage266en_US
dc.source.initialpage259en_US
dc.source.volume116en_US
dc.subjectCardenolidesen_US
dc.subjectDirect organogenesisen_US
dc.subjectFoxgloveen_US
dc.subjectGenetic stabilityen_US
dc.subjectQuality specificationen_US
dc.titleEfficient direct shoot organogenesis, genetic stability and secondary metabolite production of micropropagated Digitalis purpurea L.en_US
dc.typeArticleen_US
dc.type.article1en_US

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