Efecto del extracto etanólico de hojas de Ravenala madagascariensis Sonnerat (Strelitziaceae) en ratas Wistar gestadas y su descendencia
Date
2017-07-07
Authors
Freire Gómez, Cindy
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Central “Marta Abreu “ de Las Villas. Facultad de Ciencias Agropecuarias. Departamento de Biología
Abstract
El árbol del viajero, Ravenala madagascariensis Sonn., es ampliamente utilizado en la
medicina tradicional pero ha sido poco estudiado su efecto durante la gestación. El objetivo
del trabajo fue determinar el efecto del extracto etanólico de Ravenala madagascariensis
en ratas Wistar gestadas y su descendencia. El extracto se obtuvo mediante extracción
asistida por ultrasonido y se caracterizó físico-químicamente. Se determinó el efecto del
mismo en parámetros bioquímicos (glicemia, indicadores metabólicos en orina agua y
comida consumida, orina excretada) y morfo-fisiológicos (peso y presión arterial) en ratas
Wistar no gestadas. Además, en ratas gestadas se realizaron determinaciones de peso,
glicemia, prueba de tolerancia a la glucosa, indicadores metabólicos en sangre y orina y
parámetros reproductivos maternos. En la descendencia se cuantificó la glicemia y se
realizó el estudio morfométrico fetal y placentario. En el extracto se identificaron la
presencia de fenoles, taninos, quinonas, azúcares reductores, esteroides, alcaloides,
flavonoides, lactonas y coumarinas. En ratas no gestadas los tratamientos no modificaron
la glicemia, parámetros urinarios, cantidad de agua y comida consumida ni la presión
arterial, y no produjo anomalías en los órganos. Aumentó ligeramente el peso con las dosis
de 400 y 600 mg/kg pc; en todos los tratamientos incrementó la cantidad de orina
excretada. En ratas gestadas el tratamiento no afectó la ganancia de peso durante el
embarazo; normalizó los valores de glicemia y aumentó el perfil lipídico; mejoró la
respuesta a la prueba de tolerancia a la glucosa y mantuvo su efecto días después de su
administración; no modificó el agua consumida, la orina excretada, los parámetros
bioquímicos urinarios, ni los parámetros reproductivos maternos. En la descendencia no
modificó la glicemia, no provocó malformaciones en los fetos, aumentó significativamente
el peso de los fetos y redujo el tamaño de la placenta. El extracto etanólico de R.
madagascariensis presentó potencialidades como antihipertensivo, para aumentar el peso
de la descendencia (y en no gestantes) y como antidiabético.
The traveler tree, Ravenala madagascariensis Sonn., is widely used in traditional medicine but its effect during gestation has been little studied. The objective of this work was to determine the effect of ethanolic extract of Ravenala madagascariensis on pregnant Wistar rats and their offspring. The extract was obtained by extraction assisted by ultrasound and was characterized physico-chemically. The biochemical parameters (glycemia, metabolic indicators in urine water and food consumed, urine excreted) and morpho-physiological parameters (weight and blood pressure) were determined in unprovoked Wistar rats. In addition, determinations of weight, glucose, glucose tolerance test, metabolic indicators in blood and urine, and maternal reproductive parameters were performed in rats. In the offspring the glycemia was quantified and the fetal and placental morphometric study was performed. The extract identified the presence of phenols, tannins, quinones, reducing sugars, steroids, alkaloids, flavonoids, lactones and coumarins. In non-gestated rats the treatments did not modify glycemia, urinary parameters, amount of water and food consumed nor blood pressure, and did not produce anomalies in the organs. Slightly increased weight with doses of 400 and 600 mg / kg bw; In all treatments increased the amount of urine excreted. In rats, the treatment did not affect weight gain during pregnancy; normalized glycemia values and increased lipid profile; improved the response to the glucose tolerance test and maintained its effect days after administration; did not modify the water consumed, the urine excreted, the urinary biochemical parameters, nor the maternal reproductive parameters. In offspring, it did not change glycemia, did not cause fetal malformations, significantly increase fetal weight, and reduce placental size. The ethanolic extract of R. madagascariensis presented potentialities as antihypertensive, to increase the weight of the offspring (and in non pregnant) and as antidiabetic.
The traveler tree, Ravenala madagascariensis Sonn., is widely used in traditional medicine but its effect during gestation has been little studied. The objective of this work was to determine the effect of ethanolic extract of Ravenala madagascariensis on pregnant Wistar rats and their offspring. The extract was obtained by extraction assisted by ultrasound and was characterized physico-chemically. The biochemical parameters (glycemia, metabolic indicators in urine water and food consumed, urine excreted) and morpho-physiological parameters (weight and blood pressure) were determined in unprovoked Wistar rats. In addition, determinations of weight, glucose, glucose tolerance test, metabolic indicators in blood and urine, and maternal reproductive parameters were performed in rats. In the offspring the glycemia was quantified and the fetal and placental morphometric study was performed. The extract identified the presence of phenols, tannins, quinones, reducing sugars, steroids, alkaloids, flavonoids, lactones and coumarins. In non-gestated rats the treatments did not modify glycemia, urinary parameters, amount of water and food consumed nor blood pressure, and did not produce anomalies in the organs. Slightly increased weight with doses of 400 and 600 mg / kg bw; In all treatments increased the amount of urine excreted. In rats, the treatment did not affect weight gain during pregnancy; normalized glycemia values and increased lipid profile; improved the response to the glucose tolerance test and maintained its effect days after administration; did not modify the water consumed, the urine excreted, the urinary biochemical parameters, nor the maternal reproductive parameters. In offspring, it did not change glycemia, did not cause fetal malformations, significantly increase fetal weight, and reduce placental size. The ethanolic extract of R. madagascariensis presented potentialities as antihypertensive, to increase the weight of the offspring (and in non pregnant) and as antidiabetic.
Description
Keywords
Extractos Vegetales, Plantas Medicinales, Hipertensión, Diabetis Mellitus