Influencia del lugar de recolección sobre el contenido fenólico y las acciones farmacológicas de las hojas de Eugenia clarensis Britton & P. Wilson
Fecha
2018
Autores
León Vergel, Yainely
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Editor
Universidad Central “Marta Abreu” de Las Villas. Facultad de Química y Farmacia.Departamento de Farmacia
Resumen
Una de las especies endémicas locales de la flora Cubanacan, en la provincia de Villa Clara, destacándose sus potencialidades como antioxidante y antiinflamatorio. El presente trabajo tuvo como objetivo evaluar la influencia del sitio de colecta en la composición fenólica y la acción antiinflamatoria y antioxidante del extracto etanólico de las hojas de Eugenia clarensis. En el estudio se evaluaron muestras de tres localidades: Jardín Botánico de la Universidad Central ¨Marta Abreu¨ de Las Villas, Motembo y Los Güiros. Se evaluaron las drogas crudas secadas a través de tamizaje fitoquímico con evidencias positivas de coumarinas, triterpenos y/o esteroides, azúcares reductores, coumarinas, fenoles y/o taninos flavonoides, aminoácidos y fenoles y/o taninos. Se determinó la actividad antioxidante in vitro de los extractos etanólicos por el método de la actividad secuestradora del radical libre 2,2-Difenil-1-Picrilhidrazilo (DPPH•) y la actividad antiinflamatoria tópica in vivo a través de la técnica edema auricular inducido por acetato de tetradecanoilforbol (TPA) en ratones Balb/c hembras a la dosis 1 mg/oreja y se evaluó el contenido fenólico y de flavonoides totales. El contenido fenólico y el contenido de flavonoides resultaron variables, destacándose que los mayores contenidos de fenoles se reportaron en la muestra del Jardín Botánico, mientras que en Motembo fueron más elevados los de flavonoides. Se evidenció además, influencia sobre la actividad antirradicálica y la actividad antiinflamatoria con los mejores indicadores para el caso de la localidad de Motembo, aspecto que deberá tomarse en cuenta para futuras evaluaciones de esta especie.
The species Eugenia clarensis Britton & P. Wilson is one of the local endemics of the flora of Cubanacán located in the center of the Villa Clara province with greater potential as an antioxidant and anti-inflammatory. The objective of this work was to evaluate the influence of the collection site on the phenolic composition and the anti-inflammatory and antioxidant action of the ethanolic extract of the after of Eugenia clarensis. In the study, samples from three locations were evaluated: Botanical Garden of the Central University ¨Marta Abreu¨ of Las Villas, Motembo and Los Güiros. Crude drugs were evaluated through phytochemical screening with positive evidence of coumarins, triterpenes and / or steroids, reducing sugars, coumarins, phenols and / or flavonoid tannins, amino acids and phenols and / or tannins; while they were negative for alkaloids, saponins, mucilages and bitter principles. The in vitro antioxidant activity of the ethanolic extracts was determined by the DPPH free radical scavenger activity method and the topical antiinflammatory activity in vivo through the TPA-induced atrial edema technique in female Balb / c mice at dose 1 mg / ear and the phenolic and total flavonoid content was evaluated. The phenolic content and the flavonoid content were variable, highlighting that the highest contents of phenols were reported in the sample of the Botanical Garden, while in Motembo the flavonoids were higher. There was also evidence of an influence on antiradical activity and anti-inflammatory activity with the best indicators for the case of Motembo, an aspect that should be taken into account for future evaluations of this species.
The species Eugenia clarensis Britton & P. Wilson is one of the local endemics of the flora of Cubanacán located in the center of the Villa Clara province with greater potential as an antioxidant and anti-inflammatory. The objective of this work was to evaluate the influence of the collection site on the phenolic composition and the anti-inflammatory and antioxidant action of the ethanolic extract of the after of Eugenia clarensis. In the study, samples from three locations were evaluated: Botanical Garden of the Central University ¨Marta Abreu¨ of Las Villas, Motembo and Los Güiros. Crude drugs were evaluated through phytochemical screening with positive evidence of coumarins, triterpenes and / or steroids, reducing sugars, coumarins, phenols and / or flavonoid tannins, amino acids and phenols and / or tannins; while they were negative for alkaloids, saponins, mucilages and bitter principles. The in vitro antioxidant activity of the ethanolic extracts was determined by the DPPH free radical scavenger activity method and the topical antiinflammatory activity in vivo through the TPA-induced atrial edema technique in female Balb / c mice at dose 1 mg / ear and the phenolic and total flavonoid content was evaluated. The phenolic content and the flavonoid content were variable, highlighting that the highest contents of phenols were reported in the sample of the Botanical Garden, while in Motembo the flavonoids were higher. There was also evidence of an influence on antiradical activity and anti-inflammatory activity with the best indicators for the case of Motembo, an aspect that should be taken into account for future evaluations of this species.
Descripción
Palabras clave
Plantas Medicinales, Villa Clara, Hojas de Eugenia Clarensis, Medicina Tradicional, Farmacología, Eugenia Clarensis, Extractos Etanólicos, Antioxidantes Naturales, Medicinal Plants, Eugenia clarensis Britton, Ethanolic extracts, Anti-inflammatory Activity, Antioxidants