Obtención de un biomodelo experimental de hipertensión arterial en ratas Wistar
Date
2019-06
Authors
Cruz Cruz, José Rey
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Central "Marta Abreu " de Las Villas
Abstract
La prevalencia de la hipertensión arterial sistémica es elevada. Esta es causante de más de
doce millones de accidentes cardíacos y vasculares en el mundo cada año. La utilización de
modelos animales experimentales ha proporcionado valiosa información sobr
e muchos
aspectos de la hipertensión arterial incluyendo su fisiopatología, complicaciones y tratamiento.
Este estudio se desarrolló en la Unidad de Toxicología Experimental de Villa Clara. Se
emplearon 30 ratas de la especie
Rattus norvegicus
líne
a Wistar
.
Tuvo como objetivo obtener
un biomodelo de hipertensión arterial en ratas Wistar. Para ello se caracterizó
fisiopatológicamente el procedimiento experimental para instaurar la hipertensión arterial por
administración de solución salina y el procedimiento
experimental para instaurar la
hipertensión arterial por nefrectomía subtotal renal. Se determinaron los valores de presión
arterial sistólica y diastólica durante los días 0,7 y 15 y se extrajo sangre a los grupos
experimentales los días 0 y 15. Se evalu
aron las variables bioquímicas creatina kinasa, lactato
deshidrogenasa, Troponina T y ácido úrico y se realizó la evaluación histológica de los riñones
de todos los grupos experimentales. La retención de sodio ocasionó el aumento de la presión
arterial dur
ante los días 7 y 15, manifestando daño renal. La nefrectomía subtotal renal
aumentó la retención de sodio ocasionando el aumento de la presión arterial y el deterioro de
la función renal. Se concluyó que la retención de sodio y el daño producido por una l
esión
renal provocan el aumento de la presión arterial debido al desequilibrio de natriuresis, además
de acentuar el daño tisular.
The prevalence of systemic arterial hypertension is high. This causes more than twelve million heart and vascular accidents in the world every year. The use of experimental animal models has provided valuable information on many aspects of arterial hypertension including its pathophysiology, complications, and treatment. This study was carried out in the Experimental Toxicology Unit of Villa Clara. We used 30 rats of the Rattus norvegicus spe cies Wistar line. It aimed to obtain a bio - model of hypertension in Wistar rats. To this end, the experimental procedure to establish arterial hypertension by administering a saline solution and the experimental procedure to establish arterial hypertension by renal subtotal nephrectomy were pathophysiologically characterized. The values of systolic and diastolic blood pressure were determined during days 0,7 and 15 and blood was extracted to the experimental groups on days 0 and 15. The biochemical variable s creatine kinase, lactate dehydrogenase, troponin T and uric acid were evaluated and performed the histological evaluation of the kidneys of all the experimental groups. The retention of sodium caused the increase in blood pressure during days 7 and 15, m anifesting kidney damage. Renal subtotal nephrectomy increased sodium retention, causing an increase in blood pressure and deterioration of renal function. It was concluded that the retention of sodium and the damage produced by a kidney injury cause the i ncrease of the arterial pressure due to the imbalance of natriuresis, besides accentuating the tissue damage .
The prevalence of systemic arterial hypertension is high. This causes more than twelve million heart and vascular accidents in the world every year. The use of experimental animal models has provided valuable information on many aspects of arterial hypertension including its pathophysiology, complications, and treatment. This study was carried out in the Experimental Toxicology Unit of Villa Clara. We used 30 rats of the Rattus norvegicus spe cies Wistar line. It aimed to obtain a bio - model of hypertension in Wistar rats. To this end, the experimental procedure to establish arterial hypertension by administering a saline solution and the experimental procedure to establish arterial hypertension by renal subtotal nephrectomy were pathophysiologically characterized. The values of systolic and diastolic blood pressure were determined during days 0,7 and 15 and blood was extracted to the experimental groups on days 0 and 15. The biochemical variable s creatine kinase, lactate dehydrogenase, troponin T and uric acid were evaluated and performed the histological evaluation of the kidneys of all the experimental groups. The retention of sodium caused the increase in blood pressure during days 7 and 15, m anifesting kidney damage. Renal subtotal nephrectomy increased sodium retention, causing an increase in blood pressure and deterioration of renal function. It was concluded that the retention of sodium and the damage produced by a kidney injury cause the i ncrease of the arterial pressure due to the imbalance of natriuresis, besides accentuating the tissue damage .
Description
Keywords
Presión Arterial, Rattus Norvegicus, Retención de Sodio, Nefrectomía Subtotal Renal, Blood Pressure, Rattus Norvegicus, Sodium Retention, Subtotal Renal Nephrectomy