Caracterización de potyvirus en Dioscorea spp. en el Banco de Germoplasma de ñame del INIVIT
Fecha
2019-06
Autores
Valdés Claro, Elizabeth
Título de la revista
ISSN de la revista
Título del volumen
Editor
Universidad Central "Marta Abreu " de Las Villas
Resumen
En Cuba
, la presencia de potyvirus en el cultivo del ñame (
Dioscorea
spp.) ha aumentado
debido a que este
se ha venido propagando de forma agámica.
En este trabajo
,
se
caracterizaron los síntomas por especies y se implementaron las técnicas ELISA
-
DASI,
leaf
-
dip
y
RT
-
PCR para el diagnóstico genérico de potyvirus
y
específico del
Y
am mosaic
virus
(YMV)
y
el
Yam mild mosaic virus
(
YMMV). Se realizó un análisis filogenético de los
potyvirus encontrados y finalmente se analizaron arvenses en las áreas de producción de
semilla del INIVIT y sus alrededores. Se formaron 25 grupos síntoma
-
especie, la clorosis
extendida y el mosaico ligero son los síntomas de mayor frecuencia de aparición. Según
la prueba ELISA resultaron positivos a potyvirus 14 grupos síntoma
-
especie, dond
e
Dioscorea trifida
resulta la especie más susceptible ante l
as enfermedades virales
present
es.
En las muestras se encontraron partículas flexuosas que coinciden con el
género de los potyvirus. El RT
-
PCR confirmó la presencia de potyvirus asociados a 12 de
los 14 grupos síntoma
-
especie evaluados.
El análisis Bayesiano sugirió que han existido
multiples introducciones del YMMV en Cuba, no siendo así para el
YMV
. Se identificaron
26 especies de arvenses pertenecientes a 15 familias, 10 de las cuales resultaro
n
positivos a potyvirus según la prueba ELISA.
Las familias
Asteraceae
y
Cyperaceae
resultaron las más susceptibles ante las enfermedades virales presentes
, por lo que
e
xiste gran diversidad de adverses que constituyen hospedantes alternativos de YMV y
YMM
V
.
In Cuba, the presence of potyviruses in the cultivation of yams ( Dioscorea spp.) h as increased due to the fact that it has been spreading aggressively. In this work , the symptoms were characterized by species and the ELISA - DASI, leaf - dip and RT - PCR techniques were implemented for the generic diagnosis of potyvirus and specific ally for Yam mosaic virus (YMV) and Yam mild mosaic virus (YMMV). A phylogenetic an alysis of the potyviruses was carried out and finally weeds were analyzed in the seed production areas of INIVIT and its surroundings. Twenty - five symptom - species groups were formed, the extended chlorosis and the light mosaic are the symptoms of higher fr equency of appearance. According to the ELISA test, 14 symptom - species groups were positive for potyvirus, where Dioscorea trifida is the most susceptible species to present viral diseases. In the samples, there were flexuous particles that coincide with t he genus of the potyviruses. RT - PCR confirmed the presence of potyviruses associated with 12 of the 14 symptom - species groups evaluated. The Bayesian analysis suggested that it has been multiples introduction of YMMV in Cuba, but not for YMV . We identified 26 weed species belonging to 15 families, 10 of which were positive for potyviruses according to the ELISA test. Asteraceae and Cyperaceae were the most susceptible families to the viral diseases present , for which t here is a great diversity of adversarie s that constitute alternative hosts of YMV and YMMV.
In Cuba, the presence of potyviruses in the cultivation of yams ( Dioscorea spp.) h as increased due to the fact that it has been spreading aggressively. In this work , the symptoms were characterized by species and the ELISA - DASI, leaf - dip and RT - PCR techniques were implemented for the generic diagnosis of potyvirus and specific ally for Yam mosaic virus (YMV) and Yam mild mosaic virus (YMMV). A phylogenetic an alysis of the potyviruses was carried out and finally weeds were analyzed in the seed production areas of INIVIT and its surroundings. Twenty - five symptom - species groups were formed, the extended chlorosis and the light mosaic are the symptoms of higher fr equency of appearance. According to the ELISA test, 14 symptom - species groups were positive for potyvirus, where Dioscorea trifida is the most susceptible species to present viral diseases. In the samples, there were flexuous particles that coincide with t he genus of the potyviruses. RT - PCR confirmed the presence of potyviruses associated with 12 of the 14 symptom - species groups evaluated. The Bayesian analysis suggested that it has been multiples introduction of YMMV in Cuba, but not for YMV . We identified 26 weed species belonging to 15 families, 10 of which were positive for potyviruses according to the ELISA test. Asteraceae and Cyperaceae were the most susceptible families to the viral diseases present , for which t here is a great diversity of adversarie s that constitute alternative hosts of YMV and YMMV.
Descripción
Palabras clave
Enfermedades Virales, Dioscorea spp, Propagación Agámica, Yam Mild Mosaic Virus, Viral Diseases, Yam Mosaic Virus