Propuesta metodológica para la cuantificación de saponinas esteroidales en extractos de Agave brittoniana T
Fecha
2021
Autores
Sandelis Zorrilla, Lisandra
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Editor
Universidad Central ‘‘Marta Abreu’’ de Las Villas. Facultad de Química y Farmacia. Departamento de Farmacia
Resumen
En el trabajo de investigación se realizó una revisión bibliográfica detallada con el fin de proponer una metodología para la cuantificación de saponinas esteroidales en los extractos obtenidos de las hojas de la especie Agave brittoniana Trel. spp. Brachypus con fines farmacéuticos. En su desarrollo, se recopilaron, seleccionaron y se analizaron los resultados obtenidos de la revisión bibliográfica acerca de las metodologías analíticas descritas para la determinación cualitativa y cuantitativa de saponinas esteroidales. Se seleccionaron 31 publicaciones según los criterios de inclusión y exclusión preestablecidos. La identificación analítica se describe, mayoritariamente, por los índices de espuma y hemólisis. La técnica de cuantificación más actual es HPLC acoplado a diferentes tipos de detectores. Se recomienda para la cuantificación de saponinas esteroidales de Agave brittoniana Trel. spp. Brachypus, en muestras que requieran mayor precisión, la técnica de HPLC-UV-VIS empleando columna C-18 y fase móvil: ácido fórmico acuoso y acetonitrilo y para análisis que requieran menor precisión la técnica Espectrofotometría UV-Vis con el empleo del reactivo de Liebermann Burchard.
In the research, a detailed bibliographic review was carried out in order to propose a methodology for the quantification of steroidal saponins in the extracts obtained from the leaves of the Agave brittoniana Trel species. spp. Brachypus for pharmaceutical purposes. During its development, the results obtained from the bibliographic review about the analytical methodologies described for the qualitative and quantitative determination of steroidal saponins were collected, selected and analyzed. were selected 31 publications according to the pre-established inclusion and exclusion criteria. The analytical identification is described, mainly, by the foam and hemolysis indices. The most current quantification technique is HPLC coupled to different types of detectors. It is recommended for the quantification of steroidal saponins from Agave brittoniana Trel. spp. Brachypus, in samples that require greater precision, the HPLC-UV-VIS technique using a C-18 column and mobile phase: aqueous formic acid and acetonitrile and for analyzes that require less precision the UV-Vis spectrophotometry technique with the use of the reagent of Liebermann Burchard.
In the research, a detailed bibliographic review was carried out in order to propose a methodology for the quantification of steroidal saponins in the extracts obtained from the leaves of the Agave brittoniana Trel species. spp. Brachypus for pharmaceutical purposes. During its development, the results obtained from the bibliographic review about the analytical methodologies described for the qualitative and quantitative determination of steroidal saponins were collected, selected and analyzed. were selected 31 publications according to the pre-established inclusion and exclusion criteria. The analytical identification is described, mainly, by the foam and hemolysis indices. The most current quantification technique is HPLC coupled to different types of detectors. It is recommended for the quantification of steroidal saponins from Agave brittoniana Trel. spp. Brachypus, in samples that require greater precision, the HPLC-UV-VIS technique using a C-18 column and mobile phase: aqueous formic acid and acetonitrile and for analyzes that require less precision the UV-Vis spectrophotometry technique with the use of the reagent of Liebermann Burchard.
Descripción
Palabras clave
Agave brittoniana Trel. spp. Brachypus, Cuantificación de Saponinas Esteroidales