Caracterización de Echerichia coli (Enterobacteriaceae) involucrada en infecciones del tracto urinario en humanos.
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Fecha
2013-07-08
Autores
Venereo Concepción, Damilsa
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Universidad Central "Marta Abreu" de Las Villas
Resumen
Escherichia. coli es la enterobacteria más estudiada y por sus capacidades patogénicas ha sido sustrato y modelo de investigaciones metabólicas, genéticas y poblacionales. E. coli uropatogénica (UPEC) ha sido el término usado para describir las cepas capaces de causar infecciones del tracto urinario (ITU). La presente investigación se realizó con el objetivo de caracterizar Escherichia coli asociadas a infecciones del tracto urinario en humanos. Se realizó un estudio epidemiológico para determinar el microorganismo, edades y sexo más frecuentes en pacientes con ITU. Se determinó la resistencia antimicrobiana a los aislados de E. coli y se les realizó el test de aglutinación de levadura (Saccharomyces sereviciae) e inhibición para determinar el tipo de fimbrias que expresaban. Se demostró que el sexo femenino es el más propenso a padecer ITU (68,40%) y que las edades más frecuentes son los menores de 20 años, independientemente del microorganismo aislado. Se evidenció que E. coli es el microorganismo más abundante en pacientes con ITU, representando el 77,4% (622/803, Abril-Mayo-Jumio de 2012). Se constató que de 200 casos remitidos con posible ITU en febrero de 2013, 147 casos resultaron positivos en el urocultivo (E. coli, 68,0%; Klebsiella sp., 21,1%; Proteus mirabilis, 7,5%; Morganella morgana, 3,4%). En 100 aislados de E. coli evaluados mediante antibiograma se presentó mayor resistencia a ácido nalidíxico, ampicillín, claforán y sulfaprín. Se constató la presencia de aglutinación sensible a manosa (MS+) en 31/50 aislados de E. coli (61,0%) con elevada resistencia antimicrobiana
Escherichia. coli is the most widely studied Enterobacteriaceae and due to its pathogenic capabilities has been use as a model in ecology, enzymatic and metabolic studies. Uropathogenic Escherichia coli (UPEC) is the term used to described those strains which are capable to provoke urinary tract infections (UTI). With the aim of characterized E. coli asociated to urinary tract infection in humans was developed the present investigation. It was performed an epidemiological study to determined the most frequent microorganism, age and sex in pacients with UTI. It was stablish the antimicrobial resistance patern as well as the capacity to agglutinate yeast (Saccharomyces sereviciae) and the inhibition of agglutination to determine the fimbriae expressed by the isolates. It was demonstrated that females is more susceptible to UTI (68,40%) and that the most frequent ages are those jonger than 20 years old, unlike the kind of microorganism isolated. E. coli was the most frequent microorganism isolated from pacients with UTI being the 77,4% (622/803, April – June, 2012). It was proven that from 200 cases, suspicious to suffer UTI in February 2013, 147 cases were possitive in the Microbiology test (E. coli, 68,0%; Klebsiella sp., 21,1%; Proteus mirabilis, 7,5% Morganella morgana, 3,4%). The antibiotic resistance test in 100 E. coli isolates showed the higher resistance to nalidixic acid, ampicillin, claforan y sulphaprin. 31/50 E. coli isolates with high antimicrobial resistance (61,0%) showed mannose sensible (MS+) agglutination.
Escherichia. coli is the most widely studied Enterobacteriaceae and due to its pathogenic capabilities has been use as a model in ecology, enzymatic and metabolic studies. Uropathogenic Escherichia coli (UPEC) is the term used to described those strains which are capable to provoke urinary tract infections (UTI). With the aim of characterized E. coli asociated to urinary tract infection in humans was developed the present investigation. It was performed an epidemiological study to determined the most frequent microorganism, age and sex in pacients with UTI. It was stablish the antimicrobial resistance patern as well as the capacity to agglutinate yeast (Saccharomyces sereviciae) and the inhibition of agglutination to determine the fimbriae expressed by the isolates. It was demonstrated that females is more susceptible to UTI (68,40%) and that the most frequent ages are those jonger than 20 years old, unlike the kind of microorganism isolated. E. coli was the most frequent microorganism isolated from pacients with UTI being the 77,4% (622/803, April – June, 2012). It was proven that from 200 cases, suspicious to suffer UTI in February 2013, 147 cases were possitive in the Microbiology test (E. coli, 68,0%; Klebsiella sp., 21,1%; Proteus mirabilis, 7,5% Morganella morgana, 3,4%). The antibiotic resistance test in 100 E. coli isolates showed the higher resistance to nalidixic acid, ampicillin, claforan y sulphaprin. 31/50 E. coli isolates with high antimicrobial resistance (61,0%) showed mannose sensible (MS+) agglutination.
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Palabras clave
Escherichia Coli, Infección, Enterobacteriaceae, Sistema Urinario, Enterobacteriaceae, Sepsis, Epidemiología, Urinary Tract, Resistencia Antimicrobiana, Farmacorresistencia Microbiana, Drug Resistance, Microbial, Proteus mirabilis, Antimicrobianos, Antibióticos