Determinación de la concentración óptima de Metano Sulfonato de Etilo en Phaseolus vulgaris L. cultivar DOR 364
Fecha
2016-07-02
Autores
León García, Aidelyn
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Universidad Central "Marta Abreu" de Las Villas. Facultad de Ciencias Agropecuarias. Departamento de Agronomía
Resumen
Con el objetivo de determinar la concentración óptima de metano sulfonato de etilo
en Phaseolus vulgaris L. cultivar DOR 364 se realizó un estudio en las casas de
cultivo del Instituto de Biotecnología de las Plantas entre diciembre de 2015 y abril
de 2016. Para la inducción de mutaciones se trataron las semillas con diferentes
concentraciones de metano sulfonato de etilo (20, 30, 40, 50, 60 mM), como
control se emplearon semillas no expuestas al mutágeno. Las semillas fueron
sembradas en bolsas de polietileno con 4 kg de sustrato. Se evaluaron variables
involucradas en la germinación, el crecimiento, desarrollo foliar, morfología y
fenología de las plantas; así como los componentes del rendimiento. El metano
sulfonato de etilo influyó sobre las variables evaluadas en las distintas etapas del
cultivo. Basados en la germinación, desarrollo y crecimiento de las plantas, la
sobreviencia, los componentes del rendimiento y la frecuencia de variaciones
morfológicas y fenológicas, 30 mM de EMS es la concentración apropiada para
establecer una población de mutantes en elcultivar de frijol DOR 364, donde hasta
la fecha no se han informado estudios previos al respecto. Con esta concentración
se pudo generar el mayor número de cambios morfológicos y fenológicos con alta
eficiencia en una población pequeña.
With the objective of determining the optimum concentration of ethyl methane sulfonate in Phaseolus vulgarisL. DOR 364 cultivar. A study in the greenhouse at the Instituto de Biotecnología de las Plantas was done from December 2015 to April 2016. For mutation induction, seeds were treated with different ethyl methane sulfonate concentrations (20, 30, 40, 50, 60 mM), as control not treated with mutagen seeds were used. Seeds were sown into polyethylene bag containing 4 kg of substratum. Variables involved in germination, growing, left development, morphology and phenology of the plants, as well as, yield components were evaluated. The ethyl methane sulfonate influenced on evaluated variables at distinct crop stage. Base on germination, plant growth, overall survival, yield´s components, and morphological and phenological variations rate, 30 mM of ethyl methane sulfonate is the appropriated concentration to establish a mutant population at the common bean DOR 364 cultivar, where nowadays previous studies have not been informed regarding. With this concentration, major number of morphological and phenological changes was generated, with high efficiency in a small population.
With the objective of determining the optimum concentration of ethyl methane sulfonate in Phaseolus vulgarisL. DOR 364 cultivar. A study in the greenhouse at the Instituto de Biotecnología de las Plantas was done from December 2015 to April 2016. For mutation induction, seeds were treated with different ethyl methane sulfonate concentrations (20, 30, 40, 50, 60 mM), as control not treated with mutagen seeds were used. Seeds were sown into polyethylene bag containing 4 kg of substratum. Variables involved in germination, growing, left development, morphology and phenology of the plants, as well as, yield components were evaluated. The ethyl methane sulfonate influenced on evaluated variables at distinct crop stage. Base on germination, plant growth, overall survival, yield´s components, and morphological and phenological variations rate, 30 mM of ethyl methane sulfonate is the appropriated concentration to establish a mutant population at the common bean DOR 364 cultivar, where nowadays previous studies have not been informed regarding. With this concentration, major number of morphological and phenological changes was generated, with high efficiency in a small population.
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